Ion-pair chromatography for identification of picomolar-order protein on a PVDF membrane.

1 Amino Acid Analysis: An Overview Margaret I. Tyler ..................................................................................... 1 2 Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, and Reed J. Harris ............................................................................. 9 3 Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis Li Zhang and Nancy Denslow ............................................................ 31 4 Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Steven A. Cohen .................................................................................. 39 5 Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent) Sunil Kochhar, Barbara Mouratou, and Philipp Christen ............... 49 6 The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection C. David Forster and Charles A. Marsden ........................................ 55 7 Anion Exchange Chromatography and Intergrated Amperometric Detection of Amino Acids Petr Jandik, Christopher Pohl, Victor Barreto, and Nebojsa Avdalovic ................................................................... 63 8 Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, and Kimie Murayama ...................................................................... 87